元件指南
VectorBuilder提供了許多常用的載體元件坐頻,可供用戶在設計載體時(shí)進(jìn可呢)行選擇。下表按類型提供了這(z下和hè)些常用元件的詳細信息。
3' UTR+polyA
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
unc-54 3' UTR+polyA | Myosin-4 3' UTR 服雨with downstream sequence from 中微C. elegans | Allows transcripti是就on termination and polyadenylatio商學n of mRNA transcribed by Pol 房路II RNA polymerase; functions in somat聽對ic cells and germ cells. | Curr Biol. 18:147謝美6 (2008) | View |
tbb-2 3' UTR+polyA | Tubulin beta-2 chain 3' UTR with dow議裡nstream sequence from C. elegans | Allows transcrip這亮tion termination a兵服nd polyadenylation of mRNA transcribed 制厭by Pol II RNA polymerase; 長時functions in somatic cel購事ls and germ cells. | Curr Biol. 18:147司微6 (2008) | View |